Much of the prosecution team's hard-hitting testimony came from its star witness, Vicente Zambada Niebla.
Zambada is the son of one of the cartel's top leaders, Ismael "El Mayo" Zambada, who is considered one of Guzman's peers within the Sinaloa cartel hierarchy.
Zambada, nicknamed El Vicentillo, described in detail the exploits of the cartel in his testimony against Guzman.
In one bit of testimony, Zambada claimed Guzman had the brother of another cartel leader killed because he would not shake his hand when they met to make peace in a gang war.
"When [Rodolfo] left, Chapo gave him his hand and said, 'See you later, friend,' and Rodolfo just left him standing there with his hand extended," Zambada said, according to BBC.
The 43-year-old pleaded guilty to drug trafficking charges in Chicago in 2013, and to a trafficking conspiracy charge in Chicago days before Guzman's trial began.
Guzman's defense attorneys have argued that Zambada's father is in fact the true leader of the Sinaloa cartel.